Current Topic in Lactic Acid Bacteria and Probiotics
Korean Society for Lactic Acid Bacteria and Probiotics
Article
케피어 유래 박테리오신 생산 Enterococcus faecium 균주 선발
서숙진, 문기성*
Souk-Jin Seo, Gi-Seong Moon*
Published Online: Jun 30, 2019
Abstract
A bacteriocin producing lactic acid bacterium was isolated from kefir which is functional fermented milk. The CJNU 2008 strain was identified as Enterococcus faecium by 16S rRNA gene sequence analysis and the strain named E. faecium CJNU 2008. Antibacterial spectrum of E. faecium CJNU 2008 has shown that several strains including pathogenic bacteria Listeria monocytogenes and Staphylococcus aureus were inhibited by the cell and culture supernatant. Optimal culture conditions for production of the bacteriocin from E. faecium CJNU 2008 were MRS broth as a medium, 30°C, and pH 7.0.
Keywords: Kefir; bacteriocin; Enterococcus faecium; antimicrobial activity; Listeria monocytogenes