신생아 분변에서 분리한 Enterococcus faecalis의 내산성 및 내담즙산성 비교

김성준, 오세종^*

Comparison of Acid and Bile Tolerances for Enterococcus faecalis isolated from Infant Feces

Seongjun Kim, Sejong Oh^*

전남대학교 동물자원학부

Division of Animal Science, Chonnam National University

^*Corresponding author : Sejong Oh, Division of Animal Science, Chonnam National University, Gwangju 61186, Korea Tel: +82-62-530-2116, Fax: +82-62-530-2129, E-mail: soh@jnu.ac.kr

Copyright © Korean Society for Lactic Acid Bacteria. All rights reserved. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jun 30, 2019

Abstract

In this study, we investigated the probiotic candidates of Enterococcus faecalis isolated from infant feces. Fecal samples were inoculated on modified MRS (pH 5.4) agar and Blood-liver containing antibiotics agar. Total 10 isolates were identified through Gram stain, KOH test, catalase test, and 16S rDNA analysis. And these isolates compared to resistance against artificial gastric juice (pH 2.5, 1,000 unit pepsin) and bile salts (0.3% Oxgall). Especially, strain SJ10 survives at a level of 7.84 log CFU/ mL under 0.3% bile salts. Strains SJ1, SJ6, SJ7 and SJ10 were found to be the least acid resistance with a viable cells (SJ1: 6.97 log CFU/mL, SJ6: 7.19 log CFU/mL, SJ7: 7.02 log CFU/mL and SJ 10: 7.07 log CFU/mL, respectively). Further in vitro and in vivo studies should be evaluated with these isolates before claiming probiotic characteristics.

Keywords: Enterococcus faecalis; probiotics; bile tolerance; acid tolerance