Table 1. HPLC operating conditions for determining molecular weight distribution and sugar composition

Analysis	Molecular weight distribution	Sugar composition
Instrument	Agilent 1260 series (AgilentTechnologies,Inc., USA)	Ultimate 3000 (Dionex, Germany)
Detector	Refractive index	UV at 254 nm
Column	SuperdexTM 75 GL column (10×300 mm,GEHealthcare,USA)	YMC-Triart C18 (250×4.6 mm, 5 µm, YMCCo., Ltd., Japan)
Column temperature	Room temperature (R.T)	30°C
Flow rate	0.5 mL/min	1.0 mL/min
Mobile phase	50 mM ammonium formate buffer (pH 5.5)	0.1 M sodium phosphate buffer (pH 6.7): acetonitrile(82:18)
Injection volume	20 µL	20 µL
Data calculator	Chemstation	Chromeleon 7